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CABRI:DSMZ_MUTZ |
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Accession_number |
ACC 35
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Cell_line_name |
5637
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Brief_description |
confirmed as human with IEF of AST, MDH established from the primary bladder
carcinoma of a 68-year-old man in 1974; cells were described to produce several
growth factors (e.g. SCF, IL-1, IL-6, G-CSF, GM-CSF, etc.)
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Description |
human urinary bladder carcinoma established from the primary bladder carcinoma of
a 68-year-old man in 1974; cells were described to produce several growth factors
(e.g. SCF, IL-1, IL-6, G-CSF, GM-CSF, etc.) confirmed as human with IEF of AST, MDH
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Depositor |
Prof. K. Welte, Medical School of Hannover, Hannover, Germany
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Morphology |
epithelial-like adherent cells
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Culture_conditions |
90% RPMI 1640 + 10% FBS split confluent cultures 1:4 to 1:5 using trypsin/EDTA;
seed out at ca. 2 x 106 cells/80 cm 2 at 37C with 5% CO2 about 8-10 x 106
cells/80 cm 2
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Viruses |
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -,
HTLV-I/II -, SMRV -
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Properties |
cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +,
desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin +
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Release_conditions |
-
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Hazard |
-
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Storage |
frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule
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Doubling_time |
ca. 24 hours
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Mycoplasma |
contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI,
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Fingerprint |
multiplex PCR of minisatellite markers revealed a unique DNA profile
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Cytogenetics |
human hypotriploid karyotype with 17% polyploidy - 62(47-64)<3n>XX, -Y, -4, -5, -10,
-13, -13, -15, -18, -19, -21, -21, +4mar, der(1)t(1;?)(p21;?), der(3)t(3;?)(p22;?),
del(6)(q23), der(9)t(9;?)(p12;?), der(11)t(5;11)(q14;p12), ?der(12)t(12;?)(q14;?),
i(13q), i(14q), der(17)t(17;?)(p11/12;?), del(22)(q13) - carries large submetacentric
marker with q arm resembling tandem dup(7) - 9p12 is a recurrent breakpoint in
bladder carcinoma
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Bibliographic_reference |
Welte et al., Proc Natl Acad Sci USA, 82: 33 (1985) [PMID: 3871951
] |
Fogh, Natl Cancer Inst Monogr, 49: 33 (1978) [PMID: 571047]
Kaashoek et al., Lymphokine Cytokine Res, 10: 33 [PMID: 1883917]
Quentmeier et al., Leuk Res, 21: 33 (1997) [PMID: 9150352]
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